Composite
Part:BBa_K4329004:Design
Designed by: Yuxin He Group: iGEM22_BS_United_China (2022-09-26)
Knockdown of Bifunctional BirA enzyme
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1202
Illegal NheI site found at 4217
Illegal NheI site found at 4240 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 4264
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 49
Design Notes
We have to evaluate the off-target of the sgRNA which further can be a off-target and cause damage to the bacterial metabolic pathway.
Source
The sgRNA was designed by using CHOPCHOP.com to generate. The source of RNA promoter, dCAs9 and cas9 promoter is from BBa_K3883031.