Composite

Part:BBa_K4329004:Design

Designed by: Yuxin He   Group: iGEM22_BS_United_China   (2022-09-26)


Knockdown of Bifunctional BirA enzyme


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1202
    Illegal NheI site found at 4217
    Illegal NheI site found at 4240
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4264
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 49


Design Notes

We have to evaluate the off-target of the sgRNA which further can be a off-target and cause damage to the bacterial metabolic pathway.


Source

The sgRNA was designed by using CHOPCHOP.com to generate. The source of RNA promoter, dCAs9 and cas9 promoter is from BBa_K3883031.

References